Journal: Biology

Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions

doi: 10.3390/biology14050498

Figure Lengend Snippet: Changes in expression levels of HIF1A and RHBG in gills of Amur ide under 50 mM alkalinity treatment. * p < 0.05, *** 0.0001 < p < 0.001, **** p < 0.0001. ( A ) Relative mRNA expression levels of Hif1α after 7-day alkaline treatment; ( B ) Protein expression levels of HIF1A, RHBG, and ACTB under alkaline treatment of 0d and 3d; ( C ) Relative protein expression levels of RHBG under alkaline treatment of 0d and 3d; ( D ) Relative protein expression levels of HIF1A under alkaline treatment of 0d and 3d. Data are expressed as means ± SD ( n = 3).

Article Snippet: For immunofluorescence experiments, the slides were washed with 1 × PBS for 20 min and blocked with 5% BSA at room temperature for 1 h. Thereafter, the sections were incubated overnight at 4 °C with rabbit polyclonal antibodies against RHBG of the Amur ide and HIF1A (MCE, Kenilworth, NJ, USA, diluted 1:200).

Techniques: Expressing

Journal: Biology

Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions

doi: 10.3390/biology14050498

Figure Lengend Snippet: Expression of HIF1A and RHBG in the gills of Amur ide at 0d and 3d after treatment with 50 mM alkalinity. Scale bars are 100 μm.

Article Snippet: For immunofluorescence experiments, the slides were washed with 1 × PBS for 20 min and blocked with 5% BSA at room temperature for 1 h. Thereafter, the sections were incubated overnight at 4 °C with rabbit polyclonal antibodies against RHBG of the Amur ide and HIF1A (MCE, Kenilworth, NJ, USA, diluted 1:200).

Techniques: Expressing

Journal: Biology

Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions

doi: 10.3390/biology14050498

Figure Lengend Snippet: ( A ) The motif sequence of Hif1α obtained from the JASPAR database; ( B ) Dual-luciferase reporter assay verifying that HIF1A can bind to the Rhbg promoter. Data are expressed as means ± SD ( n = 3). The asterisk (***) denotes a significant difference.

Article Snippet: For immunofluorescence experiments, the slides were washed with 1 × PBS for 20 min and blocked with 5% BSA at room temperature for 1 h. Thereafter, the sections were incubated overnight at 4 °C with rabbit polyclonal antibodies against RHBG of the Amur ide and HIF1A (MCE, Kenilworth, NJ, USA, diluted 1:200).

Techniques: Sequencing, Luciferase, Reporter Assay